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llc cells  (ATCC)


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    ATCC llc cells
    Llc Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/llc cells/product/ATCC
    Average 99 stars, based on 1289 article reviews
    llc cells - by Bioz Stars, 2026-04
    99/100 stars

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    Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. <t>(A)</t> <t>LLC-MK2</t> cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.
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    ATCC llc cell line
    Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. <t>(A)</t> <t>LLC-MK2</t> cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.
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    ATCC llc mk2 7 1 cells
    Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. <t>(A)</t> <t>LLC-MK2</t> cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.
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    Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. (A) LLC-MK2 cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.

    Journal: bioRxiv

    Article Title: Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase protein

    doi: 10.64898/2026.03.03.709347

    Figure Lengend Snippet: Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. (A) LLC-MK2 cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.

    Article Snippet: LLC-MK2 cells (ATCC, CCL-7) were cultured in Opti-MEM I + GlutaMAX (ThermoFisher, Cat: 51985034) supplemented with 2% fetal bovine serum.

    Techniques: Binding Assay, Infection, CDC Assay, Staining, Positive Control, Negative Control, Incubation, Flow Cytometry, Standard Deviation